Enhancing the catalytic repertoire of nucleic acids: a systematic study of linker length and rigidity.

De Montfort University Open Research Archive

Show simple item record

dc.contributor.author Lee, Sarah E. en
dc.contributor.author Sidorov, Alexander en
dc.contributor.author Gourlain, Thierry en
dc.contributor.author Mignet, Nathalie en
dc.contributor.author Thorpe, Simon J. en
dc.contributor.author Brazier, John A. en
dc.contributor.author Dickman, Mark J. en
dc.contributor.author Hornby, David P. en
dc.contributor.author Grasby, Jane A. en
dc.contributor.author Williams, David M. en
dc.date.accessioned 2013-10-15T09:47:55Z
dc.date.available 2013-10-15T09:47:55Z
dc.date.issued 2001-04
dc.identifier.citation Lee, S.E. et al. (2001) Enhancing the catalytic repertoire of nucleic acids: a systematic study of linker length and rigidity. Nucleic Acids Research, 2001, 29 (7), pp. 1565-1573. en
dc.identifier.issn 0305-1048
dc.identifier.uri http://hdl.handle.net/2086/9195
dc.description.abstract The incorporation of potentially catalytic groups in DNA is of interest for the in vitro selection of novel deoxyribozymes. A series of 10 C5-modified analogues of 2′-deoxyuridine triphosphate have been synthesised that possess side chains of differing flexibility and bearing a primary amino or imidazole functionality. For each series of nucleotide analogues differing degrees of flexibility of the C5 side chain was achieved through the use of alkynyl, alkenyl and alkyl moieties. The imidazole function was conjugated to these C5-amino-modified nucleotides using either imidazole 4-acetic acid or imidazole 4-acrylic acid (urocanic acid). The substrate properties of the nucleotides (fully replacing dTTP) with Taq polymerase during PCR have been investigated in order to evaluate their potential applications for in vitro selection experiments. 5-(3-Aminopropynyl)dUTP and 5-(E-3-aminopropenyl)dUTP and their imidazole 4-acetic acid- and urocanic acid-modified conjugates were found to be substrates. In contrast, C5-amino-modified dUTPs with alkane or Z-alkene linkers and their corresponding conjugates were not substrates. The incorporation of these analogues during PCR has been confirmed by inhibition of restriction enzyme digestion using XbaI and by mass spectrometry of the PCR products. en
dc.language.iso en en
dc.publisher Oxford University Press. en
dc.title Enhancing the catalytic repertoire of nucleic acids: a systematic study of linker length and rigidity. en
dc.type Article en
dc.identifier.doi http://dx.doi.org/doi:10.1093/nar/29.7.1565
dc.funder N/A en
dc.projectid N/A en


Files in this item

Files Size Format View

There are no files associated with this item.

This item appears in the following Collection(s)

Show simple item record