High Resolution 1H NMR investigations of the capacity of dentrifices containing a smart bioactive glass to influence the metabolic profile of and deliver calcium ions to human saliva.
Dentifrices containing H2O-reactive bioactive glasses alleviate hypersensitivity in teeth via the blockage of open dentinal tubules. Here, the ability of two such products to release Ca21 ions into human saliva was investigated, together with their influence on the status of this biofluid’s 1H NMR-detectable biomolecules. Human salivary supernatants were equilibrated with increasing volumes of those derived from each dentifrice (5.00 min at 378C).These biofluids were also equilibrated at 378C with a preselected quantity of the intact products (samples were collected at increasing timepoints). Salivary Ca21 concentrations were monitored by a 1H NMR technique involving ethylenediamine tetra-acetate addition and/or atomic absorption spectrometry. Added Ca21- and dentifrice supernatant volume (DSV)- induced modifications to the salivary 1H NMR profile were explored by spectral titration. Data acquired demonstrated added DSV-dependent increases in salivary Ca21 concentrations and (Ca21-independent) modifications to the intensities of selected salivary 1H NMR signals, particularly those of the malodorous amines methyl-, dimethyl-, and trimethylamines, which were diminished by up to 80% of their prior values. Time-dependent elevations in salivary Ca21 level were observed on equilibration with the intact dentifrices. Added Ca21 ions exerted a concentration-dependent influence on a range of resonances (including those of citrate, succinate, pyruvate, and lactate). These data provide valuable information regarding the mechanisms of action of the products tested.
Citation:Grootveld, M., Silwood, C., Winter, W. (2009) High Resolution 1H NMR investigations of the capacity of dentrifices containing a smart bioactive glass to influence the metabolic profile of and deliver calcium ions to human saliva. Journal of Biomedical Materials Research Part B: Applied Biomaterials, 91B (1), pp. 88-101
- Leicester School of Pharmacy