Neural and non-neural activation of electrogenic secretion by 5-hydroxytryptamine in the rat ileum in vitro.

De Montfort University Open Research Archive

Show simple item record

dc.contributor.author Rolfe, Vivien E.
dc.contributor.author Levin, Roy J.
dc.date.accessioned 2011-11-29T12:55:20Z
dc.date.available 2011-11-29T12:55:20Z
dc.date.issued 1998
dc.identifier.citation Rolfe, V.E. and Levin, R.J. (1998) Neural and non-neural activation of electrogenic secretion by 5-hydroxytryptamine in the rat ileum in vitro. Acta Physiologica Scandinavica. 162, pp. 469-474. en
dc.identifier.uri http://hdl.handle.net/2086/5418
dc.description.abstract 5-hydroxytryptamine (5-HT) stimulates electrogenic Cl−secretion in rat ileum stripped of its outer smooth musculature and myenteric plexus. The myenteric plexus, however, is a site of 5-HT synthesis in the gut, and the plexus mediates electrogenic ion secretion activated by luminal enterotoxin STa and taurocholate. Thus, we investigated the role of the myenteric plexus in 5-HT-induced electrogenic secretion in vitro by measuring short-circuit current (Isc, microamps) with voltage-clamp apparatus as an index of electrogenic Cl−secretion in rat ileum which was either stripped of the myenteric plexus or was left intact. Serosally added 5-HT stimulated electrogenic Cl−secretion in muscle-stripped and intact ileum in a concentration-dependent manner. Pre-treatment of stripped ileum with atropine (1 μm), hexamethonium (100 μm), tetrodotoxin (1.25 μm) and capsaicin (1 μm) for 15 min did not effect the maximum Isc induced by 5-HT which would implicate a direct action on the enterocyte. In intact ilea, however, tetrodotoxin (TTX) and capsaicin reduced significantly the maximum values of Isc stimulated by 5-HT, and the nitric oxide synthase inhibitor Nω-nitro- L-arginine methyl ester ( L-NAME) caused a significant decrease in the maximum response to 5-HT. These results suggest that electrogenic secretion induced by 5-HT in rat ileum in vitro occurs partly by activation of a non-neural pathway probably involving a direct interaction with the enterocyte, and partly via a nitrinergic-myenteric secretory reflex activated by sensory afferent fibres. These data highlight the danger of characterising intestinal secretory activity from in vitro experiments by using muscle-stripped tissue only. en
dc.language.iso en en
dc.publisher Acta Physiologica Scandinavica. en
dc.subject 5-hydroxytryptamine en
dc.subject nitric oxide en
dc.subject rat ileal secretion en
dc.subject enteric nervous system en
dc.title Neural and non-neural activation of electrogenic secretion by 5-hydroxytryptamine in the rat ileum in vitro. en
dc.type Article en
dc.identifier.doi http://dx.doi.org/10.1046/j.1365-201X.1998.00309.x
dc.researchgroup Biomedical and Environmental Health
dc.peerreviewed Yes en


Files in this item

Files Size Format View

There are no files associated with this item.

This item appears in the following Collection(s)

Show simple item record