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dc.contributor.authorMasiero, Evaen
dc.contributor.authorBanik, Dipanwitaen
dc.contributor.authorAbson, Johnen
dc.contributor.authorGreene, Paulen
dc.contributor.authorSlater, A.en
dc.contributor.authorSgamma, Tizianaen
dc.date.accessioned2017-11-06T10:10:16Z
dc.date.available2017-11-06T10:10:16Z
dc.date.issued2017-10-26
dc.identifier.citationMasiero, E. et al. (2017) Genus-Specific Real-Time PCR and HRM Assays to Distinguish Liriope from Ophiopogon Samples. Plants 2017, 6 (4), 53en
dc.identifier.urihttp://hdl.handle.net/2086/14822
dc.descriptionopen access articleen
dc.description.abstractLiriope and Ophiopogon species have a long history of use as traditional medicines across East Asia. They have also become widely used around the world for ornamental and landscaping purposes. The morphological similarities between Liriope and Ophiopogon taxa have made the taxonomy of the two genera problematic and caused confusion about the identification of individual specimens. Molecular approaches could be a useful tool for the discrimination of these two genera in combination with traditional methods. Seventy-five Liriope and Ophiopogon samples from the UK National Plant Collections of Ophiopogon and Liriope were analyzed. The 5′ end of the DNA barcode region of the gene for the large subunit of ribulose-1,5-bisphosphate carboxylase/oxygenase (rbcLa) was used for the discrimination of the two genera. A single nucleotide polymorphism (SNP) between the two genera allowed the development of discriminatory tests for genus-level identification based on specific PCR and high-resolution melt curve (HRM) assays. The study highlights the advantage of incorporating DNA barcoding methods into plant identification protocols and provides simple assays that could be used for the quality assurance of commercially traded plants and herbal drugs.en
dc.language.isoenen
dc.publisherMultidisciplinary Digital Publishing Instituteen
dc.subjectOphiopogonen
dc.subjectLiriopeen
dc.subjectrbcLen
dc.subjectDNA barcodingen
dc.subjecthigh-resolution melt curve (HRM) analysisen
dc.titleGenus-Specific Real-Time PCR and HRM Assays to Distinguish Liriope from Ophiopogon Samplesen
dc.typeArticleen
dc.identifier.doihttps://dx.doi.org/10.3390/plants6040053
dc.peerreviewedYesen
dc.funderDe Montfort University HEIF funden
dc.projectidN/Aen
dc.cclicenceCC-BY-NCen
dc.date.acceptance2017-11-19en
dc.exception.reasonopen access articleen
dc.researchinstituteInstitute for Allied Health Sciences Researchen


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