Identification of G-quadruplexes in long functional RNAs using 7-deazaguanine RNA
RNA G-quadruplex (G4) structures are thought to affect biological processes, including translation and pre-mRNA splicing, but it is not possible at present to demonstrate that they form naturally at specific sequences in long functional RNA molecules. We developed a new strategy, footprinting of long 7-deazaguanine-substituted RNAs (FOLDeR), that allows the formation of G4s to be confirmed in long RNAs and under functional conditions.
Citation : Weldon, C. et al. (2017) Identification of G-quadruplexes in long functional RNAs using 7-deazaguanine RNA. Nature Chemical Biology, 13, pp. 18-20
Peer Reviewed : Yes