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dc.contributorLloyd, L. J.
dc.contributor.authorGardner, D. S.en
dc.contributor.authorDe Brot, S.en
dc.contributor.authorDunford, L. J.en
dc.contributor.authorRoma, L. G.en
dc.contributor.authorWelham, S. J. M.en
dc.contributor.authorFallman, R.en
dc.contributor.authorO'Sullivan, S. E.en
dc.contributor.authorOh, W.en
dc.contributor.authorDevonald, M. A. J.en
dc.date.accessioned2016-04-21T08:41:35Z
dc.date.available2016-04-21T08:41:35Z
dc.date.issued2015-02-15
dc.identifier.citationGardner, D.S., De Brot, S., Dunford, L.J., Roma, L.G., Welham, S.J., Fallman, R., O'Sullivan, S.E., Oh, W. and Devonald, M.A. (2015) Remote effects of acute kidney injury in a porcine model. American Journal of Physiology-Renal Physiology, 310 (4), pp. F259-F271en
dc.identifier.issn1931-857X
dc.identifier.urihttp://hdl.handle.net/2086/11959
dc.description.abstractBackground: Acute Kidney Injury (AKI) is a common and serious with no specific treatment. An episode of AKI may affect organs distant to the kidney, further increasing the morbidity associated with AKI. The mechanism of organ cross-talk after AKI is unclear. The renal and immune systems of pigs and humans are alike. Using a preclinical animal (porcine) model, we test the hypothesis that early effects of AKI on distant organs is by immune cell infiltration leading to inflammatory cytokine production, extravasation and edema. Study Design: In 29 pigs exposed to either sham-surgery or renal ischemia-reperfusion (control, n=12; AKI, n=17) we assessed remote organ (liver, lung, brain) effects in the short-(from 2 to 48h reperfusion) and longer-term (5 weeks later) using immunofluorescence (for leucocyte infiltration, apoptosis), a cytokine array, tissue elemental analysis (electrolytes), blood hematology and chemistry (e.g. liver enzymes) and PCR (for inflammatory markers). Results: AKI elicited significant, short-term (~24h) increments in enzymes indicative of acute liver damage (e.g. AST:ALT ratio; P=0.02) and influenced tissue biochemistry in some remote organs (e.g. lung tissue [Ca++] increased; P=0.04). These effects largely resolved after 48h and no further histopathology, edema, apoptosis or immune cell infiltration was noted in liver, lung or hippocampus in the short- and longer-term. Conclusions: AKI has subtle biochemical effects on remote organs in the short-term including a transient increment in markers of acute liver damage. These effects resolved by 48h and no further remote organ histopathology, apoptosis, edema or immune cell infiltration was noted.en
dc.publisherAmerican Physiology Societyen
dc.subjectremote effectsen
dc.subjectrenalen
dc.subjectacute kidney injuryen
dc.titleRemote effects of acute kidney injury in a porcine model.en
dc.typeArticleen
dc.identifier.doihttp://dx.doi.org/10.1152/ajprenal.00389.2015
dc.peerreviewedYesen
dc.funderFaculty of Medicine, Schools of Veterinary Medicine and Science and Clinical Sciences, University of Nottingham, and Nottingham University Hospitals NHS Trust Charities, Renal and Transplant Unit, City Hospital, Nottingham.en
dc.projectidN/Aen
dc.cclicenceN/Aen
dc.date.acceptance2015-11-19en


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