Dried blood spot sampling with LC-MS analysis for routine therapeutic caffeine monitoring in neonates

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dc.contributor.author Lawson, Graham en
dc.contributor.author Patel, Parul en
dc.contributor.author Mulla, Hussain en
dc.contributor.author Tanna, Sangeeta en
dc.date.accessioned 2012-11-29T16:39:01Z
dc.date.available 2012-11-29T16:39:01Z
dc.date.issued 2012-11
dc.identifier.citation Lawson, G. et al. (2012) Dried Blood Spot Sampling with LC-MS Analysis for Routine Therapeutic Caffeine Monitoring in Neonates. ISRN Chromatography, 2012, Article ID 828719 en
dc.identifier.uri http://hdl.handle.net/2086/7849
dc.description.abstract A liquid chromatography–mass spectrometry (LC-MS) method was developed and validated for the determination of therapeutic levels of caffeine in dried blood spot (DBS) samples. Caffeine is used in the treatment of Apnoea of Prematurity (AoP) in newborn children. Calibration DBS samples were prepared by spotting 15l of whole blood spiked with the analyte onto specimen collection cards. 5mm disks cut from the centre of the DBS were extracted in methanol containing the internal standard. The extract was separated using a Zorbax Eclipse Plus C18 column and the MS, operated in electrospray positive ion mode, used single ion monitoring at m/z 195 for caffeine and 198 for the IS. The overall extraction recovery of caffeine from spiked blood spots was demonstrated to be 44-47%. Validation of the micro-analytical method showed good precision (coefficient of variation) and accuracy (relative error) and specificity and was linear within the tested calibration range 500-25000ng/ml for caffeine. Investigation of different specimen collection papers revealed different matrix effects with significant ion suppression from the FTA Elute paper itself. Requiring only a micro volume (15µl) blood sample for analysis, the developed DBS based micro-analytical method has the potential to facilitate the routine monitoring of caffeine in neonates. en
dc.language.iso en en
dc.publisher International Scholarly Research Network en
dc.subject dried blood spot (DBS) en
dc.subject caffeine en
dc.subject LC-MS en
dc.subject Guthrie card en
dc.subject therapeutic drug monitoring en
dc.subject bioanalysis en
dc.subject neonates en
dc.title Dried blood spot sampling with LC-MS analysis for routine therapeutic caffeine monitoring in neonates en
dc.type Article en
dc.identifier.doi http://dx.doi.org/10.5402/2012/828719
dc.peerreviewed Yes en
dc.explorer.multimedia No en

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