Tetrahydrobiopterin regulates cyclic GMP-dependent electrogenic chloride secretion in mouse ileum in vitro.

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dc.contributor.author Rolfe, Vivien E.
dc.contributor.author Brand, Michael P.
dc.contributor.author Heales, Simon J. R.
dc.contributor.author Lindley, K. J.
dc.contributor.author Milla, Peter J.
dc.date.accessioned 2011-12-01T16:13:22Z
dc.date.available 2011-12-01T16:13:22Z
dc.date.issued 1997
dc.identifier.citation Rolfe, V.E., Brand, M.P., Heales, S.J.R., Lindley, K.J. and Milla, P.J. (1997). Tetrahydrobiopterin regulates cyclic GMP-dependent electrogenic chloride secretion in mouse ileum in vitro. Journal of Physiology, 503 (2), pp. 347-352. en
dc.identifier.issn 0022-3751
dc.identifier.issn 1469-7793
dc.identifier.uri http://hdl.handle.net/2086/5440
dc.description.abstract Basal electrogenic Cl- secretion, measured as the short-circuit current (I-SC), was variable in ileum removed from tetrahydrobiopterin (BH4)-deficient hph-1 mice and wild-type controls in vitro, although values were not significantly different.2. The basal nitrite release and mucosal cyclic guanosine 3',5'-monophosphate (cyclic GMP) production were similar in control and BH4-deficient ileum.3. Mucosally added Escherichia coli heat-stable toxin (STa, 55 ng ml(-1)) increased the nitrite release, cyclic GMP levels and the I-SC in control ileum, but its secretory actions were reduced in BH4-deficient ileum.4. L-Arginine (1 mM) increased the nitrite release, cyclic GMP production and the I-SC in control ileum, but the actions were reduced in BH4-deficient ileum.5. Serosal carbachol (1 mM) stimulated maximum short-circuit currents of similar magnitude in both control and BH4-deficient ileum, whilst nitrite release and cyclic GMP production were minimal.6. E. coli STa and L-arginine increased electrogenic Cl- secretion across intact mouse ileum in vitro by releasing nitric oxide and elevating mucosal cyclic GMP. The inhibition of these processes in the hph-1 mouse ileum suggests that BH4 may be a target for the modulation of electrogenic transport, and highlight the complexity of the interactions between nitric oxide and cyclic GMP in the gut. en
dc.language.iso en en
dc.publisher Physiological Society en
dc.subject nitric oxide en
dc.subject Escherichia Coli en
dc.subject diarrhoeal disease en
dc.subject intestinal transport en
dc.title Tetrahydrobiopterin regulates cyclic GMP-dependent electrogenic chloride secretion in mouse ileum in vitro. en
dc.type Article en
dc.researchgroup Biomedical and Environmental Health
dc.peerreviewed Yes en


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