Nitric oxide stimulates cyclic guanosine monophosphate production and electrogenic secretion in Caco-2 colonocytes.

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dc.contributor.author Rolfe, Vivien E.
dc.contributor.author Milla, Peter J.
dc.date.accessioned 2011-11-29T12:43:16Z
dc.date.available 2011-11-29T12:43:16Z
dc.date.issued 1999
dc.identifier.citation Rolfe, V.E. and Milla, P.J. (1999) Nitric oxide stimulates cyclic guanosine monophosphate production and electrogenic secretion in Caco-2 colonocytes. Clinical Science, 96 (2), pp. 165-170. en
dc.identifier.uri http://hdl.handle.net/2086/5417
dc.description.abstract Nitric oxide stimulates intestinal ion transport via the activation of enteric nerves, but it is not known whether it regulates intestinal transport function by acting on the epithelium directly. The aim of this study was to determine the influence of nitric oxide on epithelial electrogenic ion secretion, measured as the short-circuit current (Isc), using the human colonic carcinoma cell line Caco-2. The cellular mechanisms were examined by measuring epithelial cGMP production, and nitrite release was monitored as an index of nitric oxide synthesized. The nitric oxide substrate L-arginine methyl ester increased nitrite release, electrogenic secretion and cell cGMP production. Pretreatment with L-NAME (Nw-nitro-L-arginine methyl ester, 1 mM), but not the D-isomer, significantly reduced the electrogenic secretion and cGMP production evoked by L-arginine methyl ester, implicating nitric oxide synthase involvement. Pretreatment with cystamine, but not Methylene Blue, significantly reduced the maximum Isc and the cGMP release induced by L-arginine methyl ester and the nitric oxide donor sodium nitroprusside, implicating the involvement of particulate guanylate cyclase. In conclusion, nitric oxide stimulates electrogenic ion secretion and cGMP production in intestinal epithelial cells by activating particulate guanylate cyclase. The direct action of nitric oxide on the intestinal epithelium may be important in the regulation of intestinal transport function in health and in inflammatory bowel disease. en
dc.language.iso en en
dc.publisher Clinical Science en
dc.subject short-circuit current en
dc.subject L-NAME en
dc.subject Nw-nitro-L-arginine methyl ester en
dc.subject Nitric oxide en
dc.title Nitric oxide stimulates cyclic guanosine monophosphate production and electrogenic secretion in Caco-2 colonocytes. en
dc.type Article en
dc.researchgroup Biomedical and Environmental Health
dc.peerreviewed Yes en


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