Microplate spectroscopic methods for determination of the organophosphate soman.

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dc.contributor.author Prokofieva, Daria S.
dc.contributor.author Voitenko, Natalia G.
dc.contributor.author Gustyleva, Lyudmila K.
dc.contributor.author Babakov, Vladimir N.
dc.contributor.author Savelieva, E. I.
dc.contributor.author Jenkins, R. O.
dc.contributor.author Goncharov, Nikolay V.
dc.date.accessioned 2010-06-09T10:31:59Z
dc.date.available 2010-06-09T10:31:59Z
dc.date.issued 2010-12
dc.identifier.citation Prokofieva, D.S. et al (2010) Microplate spectroscopic methods for determination of the organophosphate soman. Journal of Environmental Monitoring, 12 (6), pp. 1349-1354 en
dc.identifier.uri http://hdl.handle.net/2086/3860
dc.description.abstract Two microplate spectroscopic methods for determination of organophosphates, based on inhibition of acetylcholinesterase activity, have been elaborated and evaluated for determination of the chemical weapon agent soman. The principal difference between the methods is that one measures reaction substrate concentration (elaborated from Hestrin), while the other measures reaction product (elaborated from Ellman). The linear ranges of the two methods were found to be similar. Although the limit of quantification was lower for the Ellman method (110 pM), the sensitivity coefficient was in favor of the Hestrin method (1.55-fold higher). The effects of the main soman hydrolysis products were consistent for the two methods: both methylphosphonic acid and pinacolyl methylphosphonic acid did not inhibit acetylcholinesterase activity. The main components of decontaminating solutions showed differential effects: while monoethanolamine had no influence upon results obtained by either method, hydrogen peroxide interfered with the Ellman method at far lower concentrations than with the Hestrin method. In practical applications involving samples containing hydrogen peroxide, the method based on Hestrin should be regarded as much more specific for OP determination than the Ellman method. en
dc.language.iso en en
dc.publisher The Royal Society of Chemistry en
dc.subject soman en
dc.subject organophosphates en
dc.subject acetylcholinesterase en
dc.title Microplate spectroscopic methods for determination of the organophosphate soman. en
dc.type Article en
dc.identifier.doi http://dx.doi.org/10.1039/b925815a
dc.researchgroup Biomedical and Environmental Health
dc.peerreviewed Yes en
dc.ref2014.selected 1365591152_8610680003592_3_4

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