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dc.contributor.authorKamyshny, A.en
dc.contributor.authorErmolina, I.en
dc.contributor.authorMagdassi, S.en
dc.contributor.authorFeldman, Yurien
dc.date.accessioned2017-11-22T16:02:48Z
dc.date.available2017-11-22T16:02:48Z
dc.date.issued2000-07-26
dc.identifier.citationKamyshny, A. et al. (2000) Study of the Dynamic Structure of Native and Hydrophobized Glucose Oxidase by Time-Domain Dielectric Spectroscopy. Journal of Physical Chemistry B., 104 (32), pp. 7588-7594en
dc.identifier.urihttp://hdl.handle.net/2086/14906
dc.description.abstractThe dynamic structures of native and hydrophobized (by covalent attachment of palmitoyl chains) glucose oxidase were studied by time-domain dielectric spectroscopy (TDDS). Analysis of the dipole correlation function for both types of the enzyme showed that the decay of the correlation function of the macromolecule motion can be presented as a sum of components corresponding to different kinds of protein motion:  isotropic rotation of the protein molecule as a whole, anisotropic Brownian tumbling of subunits, and anisotropic intramolecular motion of polar groups and substructures. The slowest relaxation time was found to be longer for the modified enzyme than for the native enzyme. The dielectric strengths for all relaxation processes, as well as the dipole moment and the molecular volume, were also larger for the modified glucose oxidase. The observed differences between various types of the dipole motion for the native and modified glucose oxidase are discussed.en
dc.publisherACS Publicationsen
dc.subjectHydrophobized Glucose Oxidaseen
dc.subjectmolecular dynamicsen
dc.subjectdielectric relaxationen
dc.titleStudy of the Dynamic Structure of Native and Hydrophobized Glucose Oxidase by Time-Domain Dielectric Spectroscopyen
dc.typeArticleen
dc.identifier.doihttps://dx.doi.org/10.1021/jp992454s
dc.peerreviewedYesen
dc.funderN/Aen
dc.projectidN/Aen
dc.cclicenceN/Aen
dc.date.acceptance2000-04-14en
dc.researchinstituteLeicester Institute for Pharmaceutical Innovation - From Molecules to Practice (LIPI)en


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