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dc.contributor.authorSgamma, Tizianaen
dc.contributor.authorLockie-Williams, C.en
dc.contributor.authorKreuzer, M.en
dc.contributor.authorWilliams, S.en
dc.contributor.authorScheyhing, U.en
dc.contributor.authorKoch, E.en
dc.contributor.authorSlater, A.en
dc.contributor.authorHoward, C.en
dc.date.accessioned2017-11-15T09:44:09Z
dc.date.available2017-11-15T09:44:09Z
dc.date.issued2017-10
dc.identifier.citationSgamma, T., Lockie-Williams, C., Kreuzer, M., Williams, S., Scheyhing, U., Koch, E., Slater, A. and Howard C. (2017) DNA Barcoding for Industrial Quality Assurance. Planta Medica. 83 (14/15), pp. 1117-1123en
dc.identifier.urihttp://hdl.handle.net/2086/14861
dc.description.abstractDNA barcoding methods originally developed for the identification of plant specimens have been applied to the authentication of herbal drug materials for industrial quality assurance. These methods are intended to be complementary to current morphological and chemical methods of identification. The adoption of these methods by industry will be accelerated by the introduction of DNA-based identification techniques into regulatory standards and monographs. The introduction of DNA methods into the British Pharmacopoeia is described, along with a reference standard for use as a positive control for DNA extraction and polymerase chain reaction (PCR). A general troubleshooting chart is provided to guide the user through the problems that may be encountered during this process. Nevertheless, the nature of the plant materials and the demands of industrial quality control procedures mean that conventional DNA barcoding is not the method of choice for industrial quality control. The design of DNA barcode-targeted quantitative PCR and high resolution melt curve tests is one strategy for developing rapid, robust, and reliable protocols for high-throughput screening of raw materials. The development of authentication tests for wild-harvested Rhodiola rosea L. is used as a case study to exemplify these relatively simple tests. By way of contrast, the application of next-generation sequencing to create a complete profile of all the biological entities in a mixed herbal drug is described and its potential for industrial quality assurance discussed.en
dc.language.isoenen
dc.publisherThiemeen
dc.subjectauthenticationen
dc.subjectRhodiola roseaen
dc.subjectPhyllanthus amarusen
dc.subjectOcimum tenuiflorumen
dc.subjectnext-generation sequencingen
dc.subjectmetabarcodingen
dc.subjectCrassulaceaeen
dc.subjectLamiaceaeen
dc.titleDNA Barcoding for Industrial Quality Assuranceen
dc.typeArticleen
dc.identifier.doihttps://dx.doi.org/10.1055/s-0043-113448
dc.researchgroupBiomolecular Technology Groupen
dc.peerreviewedYesen
dc.funderThe EU Seventh Framework Programme (FP7/2007 – 2013) Marie Curie Actions Industry-Academia Partnerships and Pathways schemeen
dc.projectidPIAP-GA-2011-286328 VITANGOen
dc.cclicenceN/Aen
dc.date.acceptance2017-05-27en
dc.researchinstituteInstitute for Allied Health Sciences Researchen


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