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    Browsing Leicester School of Pharmacy by Publication Date 
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    • Browsing Leicester School of Pharmacy by Publication Date
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    Browsing Leicester School of Pharmacy by Publication Date

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      • Automatic Time-Resolved Dielectric Spectrometer 

        Valitov, V. M.; Ermolina, I.; Zuev, Y.; Feldman, Yuri (Article)
      • Computer controlled system for the measurement of dielectric parameters in time domain 

        Valitov, V. M.; Ermolina, I.; Zuev, Y.; Feldman, Yuri (Article)
      • Difference method of analyzing dielectric data in the time domain 

        Feldman, Yuri; Zuev, Y.; Ermolina, I.; Goncharov, V. A. (Article)
      • NMR and dielectric spectroscopy investigation of protein dynamical structure 

        Fedotov, V.; Feldman, Yuri; Krushelnitsky, A. G.; Ermolina, I. (Article)
        The general approach to globular proteins dynamical structure investigation by NMR and time domain dielectric spectroscopy (TDDS) is presented. The information on macromolecular dynamical behavior in the case of these two ...
      • Time domain dielectric spectroscopy with nonuniform signal sampling 

        Ermolina, I.; Polygalov, E.; Romanychev, G.; Zuev, Y.; Feldman, Yuri (Article)
        The hardware and software designs for fast measurement of dielectric properties of different substances over a wide range of characteristic times (10−5–10−10 s) by means of time domain dielectric spectroscopy (TDS) are ...
      • Application of nonuniform sampling in time domain spectroscopy 

        Ermolina, I. (Article)
      • Analysis of error sources in time domain dielectric spectroscopy method 

        Romanychev, G.; Ermolina, I.; Polygalov, E.; Zuev, Y.; Ubshincki, D. V.; Feldman, Yuri (Article)
      • Lithium treatment of affective disorders; effects on the cyclic AMP and inositol phospholipid signalling pathways’ 

        Sillence, Daniel J.; Downes, C. P. (Article)
        The effects of lithium (Li+) on the adenylyl cyclase and inositol phospholipid receptor signalling pathways were compared directly in noradrenergic and carbachol stimulated rat brain cortical tissue slices. Li+ was a ...
      • Investigation of molecular motions and interprotein interactions in solutions by NMR and TDDS. 

        Ermolina, I.; Krushelnitsky, A. G.; Ivoylov, I.; Feldman, Yuri; Fedotov, V. (Article)
        Non-selective NMR relaxation of protein and water protons at various resonance frequencies as well as Time Domain Dielectric Spectroscopy (TDDS) were applied to study the molecular motions in lysozyme and myoglobin solutions. ...
      • Subcellular distribution of agonist-stimulated phosphatidylinositol synthesis in 1321 N1 astrocytoma cells 

        Sillence, Daniel J.; Downes, C. P. (Article)
        In an inositol-depleted 1321 N1 astrocytoma cell line, propranolol at 0.5 mM concentration and carbachol in the presence of Li+ induce a large increase (30-60-fold) in the amount of CMP-phosphatidate, the lipid substrate ...
      • Hydrolysis of cell surface phosphatidylinositol leads to the delayed stimulation of inositol phospholipid synthesis in Bovine Aortic Endothelial Cells 

        Sillence, Daniel J.; Low, M. G. (Article)
        In order to address the issue of how inositol phospholipid synthesis is controlled in a resting cell we looked for enhanced [3H]phosphatidylinositol (PtdIns) labelling in response to the hydrolysis of cell surface PtdIns. ...
      • Investigation of molecular motion and interprotein interactions in solutions by TDDS: A comparison with NMR data 

        Ermolina, I.; Fedotov,V.; Feldman, Yuri; Ivoylov, I. (Article)
        The results of dynamic protein behavior in solution studied by time domain dielectric spectroscopy (TDDS) are presented. The analyses for myoglobin solutions at concentrations 50, 120 and 150 mg/ml in the temperature ...
      • The dynamic behavior of myoglobin molecules in solution. 

        Nesmelova, I. E.; Ermolina, I.; Fedotov,V. (Conference)
      • Phorbol ester-sensitive phospholipase D is mainly localized in the endoplasmic reticulum of BHK cells 

        Decker, C.; Obradors, M.; Sillence, Daniel J.; Allan, D. (Article)
        The localization of phorbol ester-sensitive phospholipase D (PLD) in baby hamster kidney cells has been investigated by determining the subcellular distribution of the phosphatidylbutanol produced when the cells are incubated ...
      • Time Domain Dielectric Spectroscopy: An Advanced Measuring System 

        Feldman, Yuri; Andrianov, A.; Polygalov, E.; Ermolina, I.; Romanychev, G.; Zuev, Y.; Milgotin, B. (Article)
        The new time domain measurement system for dielectric measurements is described. The current model is comprised of an IBM PC-AT/486, ‘‘TDM-2,’’ a new time domain measurement system, a set of thermostabilized sample holders, ...
      • The use of TDDS for investigation of structural and dynamic behavior of protein molecules in aqueous solution 

        Ermolina, I.; Fedotov,V.; Feldman, Yuri (Conference)
      • Dielectric relaxation, molecular motion and interprotein interactions in myoglobin solution. 

        Ermolina, I.; Ivoylov, I.; Fedotov,V. (Article)
        The results of the investigation of protein molecule dynamic in solution by Time Domain Dielectric Spectroscopy are presented. The horse myoglobin solutions in wide range of concentration from 0.6% to 54% at 20°C have been ...
      • Evidence against an early signalling role for ceramide in Fas-mediated apoptosis 

        Sillence, Daniel J.; Allan, D. (Article)
        We have investigated whether the increases in ceramide levels that occur during apoptosis in SKW 6.4 cells induced by anti-Fas antibody depend on the activation of caspases. Using cells prelabelled to equilibrium with ...
      • The subcellular sites of sphingomyelin synthesis in BHK cells 

        Obradors, M.; Sillence, Daniel J.; Howitt, S.; Allan, D. (Article)
        The subcellular distributions of the enzymes which synthesise sphingomyelin (SM) and glucosylceramide (GluCer) from ceramide have been assessed in BHK cells. On a sucrose density gradient GluCer synthase (a marker of the ...
      • Repair of BHK cell surface ganglioside GM3 after its degradation by extracellular sialidase 

        Sillence, Daniel J.; Allan, D. (Article)
        Treatment of BHK fibroblasts with V. cholerae sialidase for 20 min caused the breakdown of about 70% of total cellular ganglioside GM3 and the production of an approximately equivalent amount of lactosylceramide. On removal ...

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