Browsing Leicester School of Pharmacy by Publication Date

Lithium treatment of affective disorders; effects on the cyclic AMP and inositol phospholipid signalling pathways’

Sillence, Daniel J. ; Downes, C. P. (Article)

The effects of lithium (Li+) on the adenylyl cyclase and inositol phospholipid receptor signalling pathways were compared directly in noradrenergic and carbachol stimulated rat brain cortical tissue slices. Li+ was a ...

Subcellular distribution of agonist-stimulated phosphatidylinositol synthesis in 1321 N1 astrocytoma cells

Sillence, Daniel J. ; Downes, C. P. (Article)

In an inositol-depleted 1321 N1 astrocytoma cell line, propranolol at 0.5 mM concentration and carbachol in the presence of Li+ induce a large increase (30-60-fold) in the amount of CMP-phosphatidate, the lipid substrate ...

Hydrolysis of cell surface phosphatidylinositol leads to the delayed stimulation of inositol phospholipid synthesis in Bovine Aortic Endothelial Cells

Sillence, Daniel J. ; Low, M. G. (Article)

In order to address the issue of how inositol phospholipid synthesis is controlled in a resting cell we looked for enhanced [3H]phosphatidylinositol (PtdIns) labelling in response to the hydrolysis of cell surface PtdIns. ...

Phorbol ester-sensitive phospholipase D is mainly localized in the endoplasmic reticulum of BHK cells

Decker, C. ; Obradors, M. ; Sillence, Daniel J. ; Allan, D. (Article)

The localization of phorbol ester-sensitive phospholipase D (PLD) in baby hamster kidney cells has been investigated by determining the subcellular distribution of the phosphatidylbutanol produced when the cells are incubated ...

The subcellular sites of sphingomyelin synthesis in BHK cells

Obradors, M. ; Sillence, Daniel J. ; Howitt, S. ; Allan, D. (Article)

The subcellular distributions of the enzymes which synthesise sphingomyelin (SM) and glucosylceramide (GluCer) from ceramide have been assessed in BHK cells. On a sucrose density gradient GluCer synthase (a marker of the ...

Evidence against an early signalling role for ceramide in Fas-mediated apoptosis

Sillence, Daniel J. ; Allan, D. (Article)

We have investigated whether the increases in ceramide levels that occur during apoptosis in SKW 6.4 cells induced by anti-Fas antibody depend on the activation of caspases. Using cells prelabelled to equilibrium with ...

Utilisation of phosphatidylcholine and production of diradylglycerol in the synthesis of sphingomyelin

Sillence, Daniel J. ; Allan, D. (Article)

After the degradation of cell-surface sphingomyelin (SM) by exogenous sphingomyelinase (SMase), the resynthesis of SM by baby-hamster kidney (BHK) and human leukaemia-60 (HL-60) cells was examined in relation to utilization ...

Repair of BHK cell surface ganglioside GM3 after its degradation by extracellular sialidase

Sillence, Daniel J. ; Allan, D. (Article)

Treatment of BHK fibroblasts with V. cholerae sialidase for 20 min caused the breakdown of about 70% of total cellular ganglioside GM3 and the production of an approximately equivalent amount of lactosylceramide. On removal ...

The synthesis of modified 5-(Aminoalkyl)- and 5-(Aminoalkenyl)- Uridine 5'-Triphosphates.

Lee, Sarah E. ; Mignet, Nathalie ; Vyle, Joseph S. ; Whetton, L. ; Grasby, Jane A. ; Williams, David M. (Article / Conference)

Recent synthetic applications of the non-classical Wittig reaction.

Murphy, Patrick J. ; Lee, Sarah E. (Article)

Sorting of cell surface components: glycolipid domains and multidrug transporters

van Meer, G. ; Sprong, H. ; Sillence, Daniel J. ; Hirabayashi, Y. ; Raggers, R. J. (Article)

Transport of (Glyco)Sphingolipids in and Between Cellular Membranes; Multidrug Transporters and Lateral Domains

van Meer, G. ; Sillence, Daniel J. ; Sprong, H. ; Kälin, N. ; Raggers, R. J. (Article)

Sphingolipids are highly enriched in the outer leaflet of the plasma membrane lipid bilayer. However, the first glycolipid, glucosylceramide, is synthesized in the opposite, cytosolic leaflet of the Golgi membrane. This ...

Assay for the transbilayer distribution of glycolipids; selective oxidation of glucosylceramide to glucuronylceramide by TEMPO nitroxyl radicals

Sillence, Daniel J. ; Raggers, R. J. ; Neville, David C. A. ; Harvey, D. J. ; van Meer, G. (Article)

In the present study, 2,2,6,6-tetramethylpiperidinooxy nitroxide (TEMPO) has been applied successfully to discriminate between glucosylceramide in the outer and inner leaflets of closed membrane bilayers. The nitroxyl ...

Apoptosis and signalling in acid sphingomyelinase deficient cells

Sillence, D. J. (Article)

BACKGROUND: Recent evidence suggests that the activation of a non-specific lipid scramblase during apoptosis induces the flipping of sphingomyelin from the cell surface to the cytoplasmic leaftet of the plasma membrane. ...

Assays for the transmembrane movement of sphingolipids

Sillence, Daniel J. ; Raggers, R. J. ; van Meer, G. (Article)

Novel syntheses of (Z)-alkene and alkane base-modified nucleosides.

Lee, Sarah E. ; Vyle, Joseph S. ; Williams, David M. ; Grasby, Jane A. (Article)

The syntheses of 5-(Z)-(3-aminoallyl)- and 5-(3-aminopropyl)-substituted 2′-deoxyuridine and 2′-deoxycytidine are reported. These compounds were derived from the corresponding 5-propargylamine derivative. [Hobbs, F. W. J. ...

EBIO, an agent causing maintained epithelial chloride secretion by co-ordinate actions at both apical and basolateral membranes.

MacVinish, L. ; Keogh, J. ; Cuthbert, A. W. (Article)

Cell cycle arrest mediated by a pyridopyrimidine is not abrogated by over-expression of Bcl-2 and cyclin D1

Soni, R. ; Chaudhuri, B. (Article)

Replication from oriP of Epstein-Barr virus requires human ORC and is inhibited by geminin